Citiwell OR7500 Manuel de service télécharger pdf (Page 14)

14 Agilent DNA 7500 and DNA 12000

5 Agilent DNA 7500 and DNA 12000 Assay Protocol

Loading the Marker

5 Press the plunger of the syringe down until it is

held by the clip.

6 Wait for exactly 30 seconds and then release the

plunger with the clip release mechanism.

7 Visually inspect that the plunger moves back at

least to the 0.3 ml mark.

8 Wait for 5 seconds, then slowly pull back the

plunger to the 1 ml position.

9 Open the chip priming station.

10 Pipette 9.0 µl of the gel- dye mix in each of the

wells marked

Loading the Marker

1 Pipette 5 µl of green- capped DNA marker vial

(green ) into the well marked with the ladder

symbol and into each of the 12 sample wells.

pressurize

9 µl gel-dye

NOTE

Protect the gel-dye mix from light. Store the gel-dye mix at 4 °C when not in use for more

than 1 hour.

5 µl marker

NOTE

Do not leave any wells empty or the chip will not run properly. Add 5 µl of green-capped

DNA marker (green ) plus 1 µl of deionized water to each unused sample well.

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Citiwell OR7500 Manuel de service Page 14